Overview of WGS Library Prep and Sequencing Options

OPTION 3

Option 3. CCGP Mini-Core

For labs that choose to use it, the CCGP offers at-cost DNA extraction and library preparation.

Approach:

• We can start with high quality tissue or high quality extracted gDNA that PIs provide

• DNA extractions will be performed robotically using standard DNA extraction kits

• Whole genome libraries will be made using the plexWell (seqWell) library preparation protocol, similar to Nextera library preparations

  • For genomes smaller than 0.5 Gb, we will use the LP 384 kit

  • For genomes larger than 0.5 Gb, we will use the WGS24 kit

• Libraries will be pooled with other CCGP projects and submitted on your behalf to a UC core

Submission:

• To submit either tissue or gDNA to the Mini-Core, please begin by filling out and submitting this submission form: https://airtable.com/shrwTLw4Z49ukKTmU

• Click here for details on submitting of tissues for DNA extraction followed by library preparation

• Click here for details on submitting gDNA for Library Preparation

• A PDF of these guidelines can also be found here: Mini-Core Submission Guidelines

Billing:

• For extraction and library preparation services, the Mini-Core can only accept funds that were distributed as part of the original CCGP award

• Sequencing services will be billed by the sequencing core for the portion of the lane(s) used (we will submit the libraries for sequencing on your behalf and include you in the process)