Mytilus californianus (California Mussel)

Visual overview of genome assembly metrics

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K-mer spectra output generated from PacBio HiFi data without adapters using GenomeScope2.0. The bimodal pattern observed corresponds to a diploid genome. K-mers covered at lower coverage but higher frequency correspond to differences between haplotypes, whereas the higher coverage but lower frequency k-mers correspond to the similarities between haplotypes. The pattern observed corresponds to a k-mer profile for a highly heterozygous species.

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BlobToolKit Snail plot showing a graphical representation of the quality metrics for the Mytilus californianus primary assembly (xbMytCali1). The plot circle represents the full size of the assembly. From the inside-out, the central plot covers length-related metrics. The red line represents the size of the longest scaffold; all other scaffolds are arranged in size-order moving clockwise around the plot and drawn in gray starting from the outside of the central plot. Dark and light orange arcs show the scaffold N50 and scaffold N90 values. The central light gray spiral shows the cumulative scaffold count with a white line at each order of magnitude. White regions in this area reflect the proportion of Ns in the assembly. The dark vs. light blue area around it shows mean, maximum and minimum GC vs. AT content at 0.1% intervals (Challis et al. 2).

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Omni-C contact map for the primary genome assembly generated with PretextSnapshot. Omni-C contact maps translate proximity of genomic regions in 3D space to contiguous linear organization. Each cell in the contact map corresponds to sequencing data supporting the linkage (or join) between two such regions. Scaffolds are separated by black lines, wherein higher density of black lines corresponds to higher levels of fragmentation.

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Authors

Lisa X Paggeot, Melissa B DeBiasse, Merly Escalona, Colin Fairbairn, Mohan P A Marimuthu, Oanh Nguyen, Ruta Sahasrabudhe, Michael N Dawson

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