Hetaerina americana (American Rubyspot Damselfly)
Visual overview of genome assembly metrics
K-mer spectrum output from PacBio HiFi data without adapters, generated using GenomeScope2.0. K-mers at lower coverage and lower frequency correspond to differences between haplotypes, whereas the higher coverage and higher frequency k-mers correspond to the similarities between haplotypes.
Snail plot showing a graphical representation of the quality metrics for the primary assembly. The circle represents the full size of the assembly. From the inside-out, the central plot displays length-related metrics. The red line represents the longest scaffold; other scaffolds are ordered by size moving clockwise around the plot and drawn in gray starting from the outside of the central plot. Dark and light orange arcs mark the scaffold N50 and N90 values. The central light gray spiral shows the cumulative scaffold count with a white line at each order of magnitude. White regions in this area reflect the proportion of Ns in the assembly The dark versus light blue area around it shows mean, maximum, and minimum GC versus AT content at 0.1% intervals (Challis et al. 2020).
Contact maps for the primary and alternate genome assembly generated with PretextSnapshot. Contact maps translate proximity of genomic regions in 3-D space to contiguous linear organization. Each cell in the contact map corresponds to sequencing data supporting the linkage (or join) between two of such regions. Scaffolds are separated by black lines and higher density corresponds to higher levels of fragmentation.
Authors
Gregory F Grether, Joscha Beninde, Eric Beraut, Noravit Chumchim, Merly Escalona, Zachary G MacDonald, Courtney Miller, Ruta Sahasrabudhe, Andrew M Shedlock, Erin Toffelmier, H Bradley Shaffer